Javier Batista Perez1
1BGI Research Foundation Latvia, Lidostas Parks, Marupe, Marupes novads, LV-2167, Latvia
javier1 [at] mgi-tech.com
Abstract
STOmics’ Stereo-seq technology is a world-leading unbiased whole transcriptome spatial omics platform that combines nanoscale resolution with the centimeter-level field of view. The Stereo-seq chip comprises billions of probes, each with spatial barcodes, in a patterned array. Biological tissue is sectioned and loaded to the Stereo-seq chip, followed by fixation and permeabilization. Then mRNA molecules from the tissue section hybridize with the barcoded probes, and reverse transcription produces spatially barcoded cDNA. Following library preparation and sequencing to generate spatially resolved Stereo-seq data from the tissue section. Proprietary cloud-based analysis reconstitutes the data and enables visualization of the transcriptomics of the original tissue section in space, empowering further research and assembly of spatial omics atlases.
Keywords: Spatial omics, single-cell unbiased whole transcriptomics, nanoscale resolution, centimeter-level field of view
